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DNA FINGERPRINTING DEMONSTRATES WIDESPREAD LACK OF ARABICA CONFORMITY

DNA FINGERPRINTING DEMONSTRATES WIDESPREAD LACK OF ARABICA CONFORMITY



Most coffee farms – and even seed lots and nurseries – do not know which varieties they are using, but there is increasing awareness that identifying optimal varieties is one of the keys to sustainable production. So, what are farmers to do? 

A new, open-access study published in the Journal of AOAC International describes a way to authenticate Arabica coffee varieties using ‘SSR DNA’ fingerprinting and describes how the method can help move the needle toward a more professional seed sector. 

The method has been used by World Coffee Research (WCR) on more than 2,500 coffee samples from farms, seed lots, and nurseries around the world. The authors confirm that – depending on the variety – genetic conformity ranges from under 40 per cent (for a Gesha) to over 90 per cent (for Marsellesa). 

The study’s authors write, “DNA fingerprinting provides different actors in the coffee sector with a powerful new tool.

“Farmers can verify the identity of their cultivated varieties, coffee roasters can be assured that marketing claims related to varieties are correct, and most of all, those looking to establish the a more professional and reliable coffee seed sector have a reliable new monitoring tool to establish and check genetic purity of seed stock and nursery plants.”

The authors of the study are Solene Pruvot-Woehl (WCR), Sarada Krishnan (Denver Botanic Garden), William Solano (CATIE), Tim Schilling (WCR), Lucille Toniutti (WCR), Benoit Bertrand (CIRAD), and Christophe Montagnon (RD2 Vision).

In many coffee-producing countries the widespread cultivation of improved varieties is extremely low, with the notable exceptions of Colombia and Honduras (for Arabica), and Vietnam (for Robusta), where significant renovation and replanting schemes have taken place. 

Even in major or well-known producing countries like Brazil or Costa Rica, most coffee land is still cultivated using varieties selected in the 1950s, such as Caturra, Catuai or Mundo Novo. Because of past limited appetite for improved varieties, the coffee seed sector has remained poorly organized in most parts of the coffee world. 

The study’s authors find the genetic conformity of coffee material – on farms, in seed lots, and in nurseries – is frequently questionable, likely due to the lack of order and good practices in the coffee seed sector (the network of systems that propagates new coffee plants and gets them into the hands of farmers). 

After decades of informal seed exchange, sometimes over borders, it is very difficult to ascertain the true-to-type-ness of cultivated varieties, creating a major barrier to getting improved materials into the hands of farmers. 

This is a concern when improved varieties are increasingly acknowledged to be essential for a sustainable coffee industry, namely for disease resistance, adaptation to climate change and market demand for high quality coffees. 

The study finds that a significant share of cultivated Arabica coffee trees in the field are mixes derived from one or several generations of uncontrolled pollinations between existing varieties. This is the case in Central America for Catimors and Sarchimors. 

In East Africa, ‘true’ SL28 and SL34 are widespread, but so are populations that evolved from varieties like the SLs and K7, which were initially well defined, but have drifted. 

Samples of Gesha, one of the most famous coffee varieties in the world, matched the ‘reference’ variety only 39 per cent of the time. 

Experience with the WCR genetic database points to the conclusion that a recently-selected variety in a region with a relatively organized research and nurseries network exhibit higher genetic conformity. 

The best example of this currently is the Marsellesa variety, with 91 per cent genetic conformity. However, when varieties are older and/or the research and nurseries network is poorly organized, the percentage of genetic conformity can drastically decrease.

“In order for coffee producers to benefit from genetic improvement, and to meet the growing demand for specific varieties, there is need to professionalize the coffee seed sector,” said WCR.

“This DNA fingerprinting method provides nurseries, farmers and the whole coffee industry with a unique opportunity to increase knowledge about the genetic identify of trees that are planted or seeds that are traded. The study’s results show that most varieties can be easily identified through SSR-based DNA fingerprinting methods.”

In 2017, World Coffee Research launched a DNA fingerprinting service for arabica coffee. The service is available to nurseries, coffee farmers, green coffee suppliers, and roasters for the authentication of arabica coffee varieties. 

The service costs US$130/sample for either tree leaves, green coffee, or roasted coffee.  Email dna@worldcoffeeresearch.org for more information. 

Read the full study in the Journal of AOAC International.

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